University Of California Los Angeles Work Values

Daily Duties at University Of California Los Angeles:

• Handle a research project that focus on understanding the relationship of proteolysis of mitochondrial proteases to the Parkinson’s disease using proteomics approaches • Provide technical support and offer research ideas to all graduate students and junior postdoctoral fellows for their projects

What they like about University Of California Los Angeles:

A hiring company that offers alternative approaches to pay, performance bonuses, and/or equity in the firm is very attractive to you. You're generally less interested in working for an organization offering only traditional, salary-based compensation, one with limited opportunity to acquire equity in the firm, or one that does not actively promote skill development. In addition, you are more likely to choose a company that offers a variety of internal career options, with defined career paths. You view the learning of new skills and development of your expertise as key to your career advancement. These aspects of an organization may become even more important to you as you progress in your field, and are especially critical if you change career or occupation.

Tags

proteomics, peptide, protein characterization, protein/antibody purification, bioassay, LCMS

Skills

• Skilled at performing LCMS (ion trap, nano-LC-LTQ and nano-LC-LTQ-FTICR), confocal, and multiphoton microscope imaging, coupled with expertise in operating surface plasmon resonance (SPR, BiaCore), spectrofluorimeter, and plate readers., • Exemplify proven success in conducting protein expression in insect, mammalian, and bacterial systems as well as purification of protein/antibody using gel filtration, ion exchange, hydrophobic, and affinity chromatography with AKTA FPLC system , • Strong ability in peptide/protein characterization using mass spectrometry and proteomics database search engines, both completed in fiercely competitive and fast-paced environment, • Proficient in preparing samples for proteomics using 1-D, 2-D gel electrophoresis, tryptic digestions, and western blotting, • Offer unyielding expertise in molecular biology (PCR, construct preparation and cloning), qPCR, siRNA and isolation, and purification of DNA and RNA, • Possess in-depth knowledge of high-throughput assay development, including ELISA and enzyme assays, cell-based real time activity assays, fluorescence ligand binding assays, mass spectrometric assays, and radio ligand binding assays, • Acquire thorough knowledge of mammalian cell culture and transfection, • Expertly dealt with estrogen, nitric oxide, eicosanoid, apoptotic, and chemoprevention signaling pathways, • Display technical skills in MassMatrix, Mascot, PyMol, Microsoft Office Suite, ChemDraw, and Sigma plot

These are some of the questions we asked our climbers about their experiences with University Of California Los Angeles: