National Institute Of Environmental Health Sciences Work Values

Daily Duties at National Institute Of Environmental Health Sciences:

*develop and maintain Genetic Mouse models (in vivo model) in cluding ► care and breeding ►genotyping ► characterisation and utilize ► injection ► immunization ► dissection ► cell isolation *Cell culture analyses of ► mammalian cell lines and ► primary cells (including ► transfection ► stimulation ► cell assays) *analyses at the molecular level of knock out cells (including ► Plasmid purification ► DNA / RNA isolation ► cDNA conversion ► DNA / siRNA-Transfection ► TaqMan RT-PCR (TaqMan® real-time PCR/qRT-PCR) ► RT-PCR (Real-time PCR) ► PCR (polymerase chain reaction) ► Site-directed mutagenesis ► Sequencing ► * Protein Biochemistry (Biochemical/Cellular Assays): ► Handle immunohistochemistry (IHC) ► immunoprecipitation (IP) ► SDS-PAGE ► native gel ► Western blot ► protein over expression and purification (bacterial- and mammalian recombinant protein expression) ► biochemical characterization of proteins (phosphorylation, sumoylation) ► in vitro caspase cleavage assay ► colorimetric GTPase assay ► cytokine-assay/enzyme-linked immunosorbent assay (ELISA). * Molecular Biology: ► Obtain experience on plasmid purification ► DNA/RNA isolation ► cDNA conversion ► DNA/siRNA-Transfection ► TaqMan RT-PCR (TaqMan® real-time PCR/qRT-PCR) ► RT-PCR (Real-time PCR) ► PCR (polymerase chain reaction) ► site-directed mutagenesis ► sequencing ► CHIP-assay (Chromatin Immunoprecipitation) * Microscopy: ► Administer fluorescence/confocal microscopy. • teaching at the NIEHS Summer Internship Program 2011 (lecture series within the Summers of Discovery (SOD) program) • mentored undergraduate students; supervised and designed student research projects

What they like about National Institute Of Environmental Health Sciences:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

Genetic Mouse Models (In Vivo Model), Cell Biology (Ex Vivo / In Vitro), Immunology, Protein Biochemistry (Biochemical/ Cellular Assays), Molecular Biology, Microscopy, Industry, Research Scientist, Investigator, Cell culture, signal transduction, signal pathway, RT-PCR, molecular cloning, fluorescence-activated cell sorting FACS, apoptosis, proliferation, immunohistochemistry, immunoprecipitation, SDS-PAGE, Western blot, proteinexpression, mammalian, enzyme-linked immunosorbent assay (ELISA), TaqMan RT-PCR, siRNA, site-directed mutagenesis, CHIP-assay, lymphocytes, immune system, RNA isolation, cDNA conversion, Protein Purification, Toxicology, Proteomics, Protocol Development, primary cells, blood cancer, infection, inflammation, cell assay, multi-task

Skills

P&L responsibilities, Presentation, Ecommerce Strategy and digital marketing channels optimization - SEO, SEM, email, affiliates - for high-profile accounts., Technology Solutions, Sales Support, Software Implementation, Requirements Analysis & Specification, Leadership

These are some of the questions we asked our climbers about their experiences with National Institute Of Environmental Health Sciences: