Dept. Of Ophthalmology Harvard Medical School Work Values

Daily Duties at Dept. Of Ophthalmology Harvard Medical School:

Genetic mechanisms of retinal development and associated macular degeneration. • Large-scale genetic mutagenesis to identify mutations that cause macular degeneration during development in zebrafish. • Genetic mapping, positional cloning and characterization of responsible gene for these defects. (3) Mechanistic and genetic screening on clinical samples to identify genetic defects causing macular degeneration in human. •Present research at conferences at Society of Developmental Biology and Zebrafish Meeting on Genetics and Development. • Preparation of manuscripts for publication in academic journals.

What they like about Dept. Of Ophthalmology Harvard Medical School:

You give high priority to hiring firms with prestige. Market reputation and prestige of a firm may be determined through on-line research, and conversations with individuals working in your target industry. Note that firms "in the public eye" receive a great deal more critical publicity and attention than those operating with more anonymity. So as you assess your employment opportunities, be sure to seek out the positive aspects of the firm's reputation in addition to noting negative publicity the company may have received.

Tags

research and development, life scientist, molecular biology, neuroscience and pain, genetics, cell biology, developmental biology, neurobiology, gene therapy

Skills

• Gene expression vector cloning, site-targeted deletion and mutagenesis, genotyping, genetic mapping and positional cloning of diseases genes, nucleic acid sample preparation, DNA sequencing. , • Luciferase assay, in situ hybridization, siRNA knockdown, promoter transcriptional activity assay. RT-PCR, single cell RT-PCR, realtime RT-PCR. , • GST-pull down, co-immunoprecipitation, chromatin-immunoprecipitation, Western blot, affinity purification of antibody, mass spectrometry and immunohistochemistry. , • Transgenic, knockout or mutant animal breeding, handling and genotyping., • Optic nerve injury, peripheral nerve injury, spinal cord injury, and neuropathic pain models., • ENU mutagenesis and mutant identification, microinjection, making transgenic lines and mosaic transplantation in zebrafish. , • Primary explants cultures of dorsal root ganglia, hippocampal neurons, and dorsal root ganglia (DRG) neurons., • Stable cell line generation and cell culture. , •Plasmid and siRNA transfection, viral vector infection., • TUNEL assays, Caspase detection assays, MTT assay, Flow cytometry, ELISA. , • Scanning and transmission electron microscopy; light, fluorescent, and confocal microscopy., • Cell division and migration live imaging in transgenic zebrafish., • Calcium imaging of primarily cultured neurons.

These are some of the questions we asked our climbers about their experiences with Dept. Of Ophthalmology Harvard Medical School: