University Of Pittsburgh Work Values

Daily Duties at University Of Pittsburgh:

Primary research interest is to identify and characterize potential therapeutic treatments for neural degeneration following injury and related disorders, including pain. And explore the mechanism and function of Sox11 in nerve regeneration.

What they like about University Of Pittsburgh:

Working in an organization that is technologically advanced, creative, and innovative is critical in your assessment of hiring companies. Less important to you are stability of the organization, the length of time an organization has been in business, and the business' plans for the future. You prefer a fast-moving company that will take risks to achieve its goals and objectives. The length of time the company has been in business is irrelevant. Maintaining status quo is unnecessary. You thrive on change, uncertainty and the upside of potential business risks, especially those associated with innovation. Stability for the long haul is not nearly as important as is working in an atmosphere that is charged with a sense of urgency and constant change.

Tags

research and development, life scientist, molecular biology, neuroscience and pain, genetics, cell biology, developmental biology, neurobiology, gene therapy

Skills

• Gene expression vector cloning, site-targeted deletion and mutagenesis, genotyping, genetic mapping and positional cloning of diseases genes, nucleic acid sample preparation, DNA sequencing. , • Luciferase assay, in situ hybridization, siRNA knockdown, promoter transcriptional activity assay. RT-PCR, single cell RT-PCR, realtime RT-PCR. , • GST-pull down, co-immunoprecipitation, chromatin-immunoprecipitation, Western blot, affinity purification of antibody, mass spectrometry and immunohistochemistry. , • Transgenic, knockout or mutant animal breeding, handling and genotyping., • Optic nerve injury, peripheral nerve injury, spinal cord injury, and neuropathic pain models., • ENU mutagenesis and mutant identification, microinjection, making transgenic lines and mosaic transplantation in zebrafish. , • Primary explants cultures of dorsal root ganglia, hippocampal neurons, and dorsal root ganglia (DRG) neurons., • Stable cell line generation and cell culture. , •Plasmid and siRNA transfection, viral vector infection., • TUNEL assays, Caspase detection assays, MTT assay, Flow cytometry, ELISA. , • Scanning and transmission electron microscopy; light, fluorescent, and confocal microscopy., • Cell division and migration live imaging in transgenic zebrafish., • Calcium imaging of primarily cultured neurons.

These are some of the questions we asked our climbers about their experiences with University Of Pittsburgh: