University Of Pittsburgh Work Values

Daily Duties at University Of Pittsburgh:

Primary research interest is to identify and characterize potential therapeutic treatments for neural degeneration following injury and related disorders, including pain. And explore the mechanism and function of Sox11 in nerve regeneration.

What they like about University Of Pittsburgh:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

research and development, life scientist, molecular biology, neuroscience and pain, genetics, cell biology, developmental biology, neurobiology, gene therapy

Skills

• Gene expression vector cloning, site-targeted deletion and mutagenesis, genotyping, genetic mapping and positional cloning of diseases genes, nucleic acid sample preparation, DNA sequencing. , • Luciferase assay, in situ hybridization, siRNA knockdown, promoter transcriptional activity assay. RT-PCR, single cell RT-PCR, realtime RT-PCR. , • GST-pull down, co-immunoprecipitation, chromatin-immunoprecipitation, Western blot, affinity purification of antibody, mass spectrometry and immunohistochemistry. , • Transgenic, knockout or mutant animal breeding, handling and genotyping., • Optic nerve injury, peripheral nerve injury, spinal cord injury, and neuropathic pain models., • ENU mutagenesis and mutant identification, microinjection, making transgenic lines and mosaic transplantation in zebrafish. , • Primary explants cultures of dorsal root ganglia, hippocampal neurons, and dorsal root ganglia (DRG) neurons., • Stable cell line generation and cell culture. , •Plasmid and siRNA transfection, viral vector infection., • TUNEL assays, Caspase detection assays, MTT assay, Flow cytometry, ELISA. , • Scanning and transmission electron microscopy; light, fluorescent, and confocal microscopy., • Cell division and migration live imaging in transgenic zebrafish., • Calcium imaging of primarily cultured neurons.

These are some of the questions we asked our climbers about their experiences with University Of Pittsburgh: