University Of The Witwatersrand Work Values

Daily Duties at University Of The Witwatersrand:

Podophyllotoxin memitic scaffolds: Structural simplification of an antimitotic natural product podophyllotoxin with memitic heterocyclic scaffolds constructed using multicomponent reactions led to the identification of compounds exhibiting low nanomolar antiproliferative and apoptosis inducing properties. The most potent compounds were found in the dihydropyridopyrazole, dihydropyridonaphthalene, dihydropyridoindole and dihydropyridopyrimidine scaffold series. Mechanistic studies performed with dihydropyridopyrazole compounds showed that these heterocycles inhibit in vitro tubulin polymerization and disrupt the formation of mitotic spindles in dividing cells at low nanomolar concentrations, in a manner similar to podophyllotoxin itself. Separation of a racemic dihydropyridonaphthalene into individual enantiomers demonstrated that only the optical antipode matching the absolute configuration of podophyllotoxin possessed potent anticancer activity. Computer modeling, performed using the podophyllotoxin binding site on beta-tubulin provided a theoretical understanding of these successful experimental findings. Crystallographic studies were performed on these new scaffolds. Concentrations (nM) required to reduce the viability of HeLa (c) and MCF-7 (d) cells by 50% after 48 h of treatment with indicated compounds, relative to DMSO control;  SD from two independent experiments, each performed in 8 replicates, determined by MTT assay. The evaluation of these compounds for antiproliferative activity against HeLa and MCF-7 cells indicated that they are more potent than podophyllotoxin itself and many of them have GI50 values as low as 3 nM. The addition of a hydroxyl group to the naphtholic moiety had a beneficial effect by further lowering the GI50 values, which is an excellent finding considering the potential water solubility problems associated with these hydrophobic compounds. These memitic heterocyclic scaffolds are considered for the clinical trials.

What they like about University Of The Witwatersrand:

Organizations with strong, centralized leadership are particularly attractive to you. You require a work environment with leadership that aggressively seeks to expand and grow the business and does so in a visible and decisive manner. In general you prefer to work in an environment in which there is a strong link between leadership, its actions, and a strong set of company-wide values.

Tags

synthetic chemist, medicinal chemist, lab technician, QC chemist, Analytical chemist

Skills

P&L responsibilities, Presentation, Ecommerce Strategy and digital marketing channels optimization - SEO, SEM, email, affiliates - for high-profile accounts., Technology Solutions, Sales Support, Software Implementation, Requirements Analysis & Specification, Leadership , Planning and executing organic synthesis in stipulated time, World wide literature survey , Purification, isolation of the crude compounds using chromatographic techniques, Use of HPLC, GC-MS, UV, NMR, FTIR for characterizing the compounds, Interpretation of the spectral data, proposing the mechanisms for product and byproduct formation, Microsoft Word, Excel, Chemdraw package , Writing the manuscripts, power point presentations, Omega software, LIMS, Speaker experience on hte world stage, Reviewing the manuscripts, Completing the projects within holding times to the customer satisfaction with high productivity

These are some of the questions we asked our climbers about their experiences with University Of The Witwatersrand: