Johns Hopkins School Of Medicine Work Values

Daily Duties at Johns Hopkins School Of Medicine:

I scientifically and financially managed a small research lab within the dermatology department. Our group was involved in two major research projects: (1) Role of midkine network in pathogenesis of NF1 and (2) biochemistry and molecular biology of p63 signaling in pathogenesis of ectodermal dysplasia. My particularly strong background in molecular biology and proteomics allowed me to apply these methods to analyze complex inherited disorders and identify specific molecular interactions associated with mutated proteins. In the past four years, our group published about 8 papers in peer review journals such as JBC, Nature Medicine, Cell Cycle, Cancer Cell and Cancer Research with high citation indexes. I was awarded two research grants. Moreover, we discovered the molecular mechanism for Hay Wells syndrome, a complex genetic disorder associated with aberrant splicing of the FGFR2 receptor, consequently linking p63 transcription, signaling and mRNA processing. During my tenure at the dermatology department, I learned many new techniques including: allele-specific "knock in" of p63 mutations in ES mouse cells, screening and propagation of ES cells, maintaining Chimera mice and establishing mice colonies, expression of heterological proteins (splicing and transcription factors) in adenoviral vectors, targeted "knockout" of receptor genes by shRNAi expressed

What they like about Johns Hopkins School Of Medicine:

The social vibrance of a hiring firm is very important to you. Your ability to make and maintain friendships there is a critical part of your decision. You would likely be dissatisfied with a workplace that is quiet, cold, or otherwise not particularly social. When you investigate a new hiring company, ask recruiters, managers, and potential co-workers about the social life and opportunities there. This is especially important when you are relocating; moving dramatically alters your social sphere both inside and outside the workplace.

Tags

scientist Ph.D molecular biology

Skills

P&L responsibilities, Presentation, Ecommerce Strategy and digital marketing channels optimization - SEO, SEM, email, affiliates - for high-profile accounts., Technology Solutions, Sales Support, Software Implementation, Requirements Analysis & Specification, Leadership , FPLC protein purification , Yeast and mammalian two-hybrid screening , Cell cultures, primary and cell lines , Confocal and phase contrast microscopy , Establishing stable cell lines , Expression proteins in adenoviral vectors , Expression shRNA in retroviral vectors , Grant and project proposal writing , Immunoprecipitation assay , Mascode analysis , Microsoft Office (Word, Excel, PowerPoint, Adobe acrobate and photoshop , MS-MALD analysis , Affimetrix expression profiling , PCR and RT-PCR amplification , Phage display screening , Random and site-specific mutagenesis , SDS-PAGE, 2-D PAGE , Sequencing of DNA and sequence analysis , Targeted knock out of genes in bacterium , Targeted knock out of genes in ES cells , Vector design and DNA cloning , Vector NTI, LaserGene, Internet databases

These are some of the questions we asked our climbers about their experiences with Johns Hopkins School Of Medicine: